| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 421 | Methanosphaera cuniculi | TGGAACAACCGCAAGTTCAA | GCTTCAACTCCAGGACTAGCA | 58.54 | 59.72 | 168 | 74 |
100.00%
|
100.00%
|
| Primer 422 | Methanosphaera cuniculi | TGGAACAACCGCAAGTTCAA | TGCTTCAACTCCAGGACTAGC | 58.54 | 59.72 | 169 | 74 |
100.00%
|
100.00%
|
| Primer 423 | Methanosphaera cuniculi | TCATGGGTCCGGTTGCAA | ACCCAGTGCTACTCCAAATGA | 59.16 | 59.01 | 257 | 74 |
100.00%
|
100.00%
|
| Primer 424 | Methanosphaera cuniculi | ATCATGGGTCCGGTTGCAA | ACCCAGTGCTACTCCAAATGA | 59.62 | 59.01 | 258 | 74 |
100.00%
|
100.00%
|
| Primer 425 | Methanosphaera cuniculi | GCAGCAATGGAACTACCGG | AGGGTCTCTATGCCATAGTGG | 58.90 | 58.39 | 216 | 74 |
100.00%
|
100.00%
|
| Primer 426 | Methanosphaera cuniculi | TGCAGCAATGGAACTACCGG | AGGGTCTCTATGCCATAGTGG | 60.68 | 58.39 | 217 | 74 |
100.00%
|
100.00%
|
| Primer 427 | Methanosphaera cuniculi | ACCACATGCATGTACTGGTGA | TGCCCTCAGCTTCCACAA | 59.65 | 58.75 | 182 | 74 |
100.00%
|
100.00%
|
| Primer 428 | Methanosphaera cuniculi | TGCTCACCATGGCGTGAT | TTGCACGTCTTGATTCACGAC | 59.33 | 59.47 | 175 | 74 |
100.00%
|
100.00%
|
| Primer 429 | Methanosphaera cuniculi | CGTAGCAGCACACGAAGGA | AGGTGACCGGTGTTCCAA | 60.08 | 58.35 | 240 | 74 |
100.00%
|
100.00%
|
| Primer 430 | Methanosphaera cuniculi | ACCTGTCACACCAGCAAGT | TTTGAGCTACTCCAACCGGA | 59.08 | 58.66 | 245 | 73 |
100.00%
|
100.00%
|